Coding

Part:BBa_K5048045:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


ccdB-dt-Plux-ccdA-dt-J23106-LuxR-dt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 897
    Illegal NheI site found at 920
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.


Source

complete synthesis


References